Journal: Cells
Article Title: Oversecretion of CCL3 by Irradiation-Induced Senescent Osteocytes Mediates Bone Homeostasis Imbalance
doi: 10.3390/cells14040249
Figure Lengend Snippet: Differential gene expression and signaling pathway enrichment in irradiated OCYs. ( A ) Volcano plots depicting the differential gene expression based on our RNA sequencing analysis. Thirty three significantly upregulated genes are represented in red points, while 74 downregulated genes are marked as orange points. ( B ) A heatmap of the hierarchical clusters of 37 differentially expressed genes in irradiated OCYs, with the black arrow indicating the position of CCL3 within the heatmap. ( C ) A gene ontology (GO) enrichment analysis showing significantly enriched biological functions in the irradiated OCYs. ( D ) A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealing the significantly enriched signaling pathways. Genes with an adjusted P-value of less than 0.05 were considered differently expressed, and biological functions or pathways with an adjusted Q-value of less than 0.05 were regarded as significantly enriched.
Article Snippet: High-throughput RNA sequencing (RNA-seq) and transcriptome analysis were performed by Gene Denovo Biotechnology Co., Ltd. (Guangzhou, China), following previously reported protocols [ ].
Techniques: Gene Expression, Irradiation, RNA Sequencing, Protein-Protein interactions